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                微波_超聲協同輔助提取靈芝多糖工藝

                52
                2010, Vol. 31, No. 16
                食品科學
                ※工藝技術
                微波 - 超聲協同輔助提取靈芝多糖工藝
                黃生權 1 , 2 ,李進偉 3 ,寧正祥 1 , *
                (1.華南理工大學輕工與食∮品學院,廣東 廣州 510640;2. 無限極(中國)有限公司,廣東 江門 214122) 529156; 3. 江南大學食品學院,江蘇 無錫
                摘 要:分析不品对付妖兽根本不是问题種和來源的 5 種靈芝♂的主要成分,確定浙江赤靈芝作為提取靈芝多糖的較佳原料。采用⊙響應面 優化超聲 - 微波協同萃取法提取靈芝多糖的最佳工藝條件@,分析超聲 - 微波協同萃取法對靈芝纖还真有点野兽維結構的影響,比 較傳統水浴浸提@ 法和超聲 - 微波協同萃取法對靈所以西蒙直到今天才再次与他相遇芝多糖提取率和結構的◆影響。結果表明:超聲 - 微波協同萃取最佳 的提取︻工藝條件為原料用量 100g,微波功率 284W,提取時間 12min,料液比(g/mL)1:11.6;與傳統水浴现在完全是处于劣势浸提法相 比,超聲 - 微波協同萃取法在較短的超聲提取時間下,靈芝多糖的提取率從 1.517% 提高到了 3.27%。 關鍵詞:超聲 - 微波協同萃取;傳統水浴浸提法;靈芝多糖;提取率
                Ultrasonic-microwave Synergistic Extraction of Polysaccharides from Cultivated Ganoderma lucidum
                HUANG Sheng-quan 1,2,LI Jin-wei3,NING Zheng-xiang 1, * (1. College of Light Industry and Food Science, South China University of Technology, Guangzhou Co. Ltd., Jiangmen 510640, China ; 2. Infinitus 214122, China) 529156, China ;3. School of Food Science and Technology, Jiangnan University, Wuxi
                Abstract :Different cultivated species of Ganoderma from different geographic origins were analyzed for their chemical components, and Ganoderma lucidum from Zhejiang province was found to contain higher content of non-reducing sugars and lower contents of protein and ash and therefore, it was the selected raw material for extracting polysaccharides. The extraction process with water as extraction solvent on an ultrasonic-microwave synergistic extractor was optimized using response surface methodology based on Box-Behnken experimental design. Meanwhile, the structural change of Ganoderma lucidum before and after extraction was observed under scan electron microscope (SEM) and the extraction method developed in this study and the conventional water bath extraction method were compared for differences in the extraction yield of polysaccharides and the infrared spectral characteristics of the extraction products. The optimum conditions for the ultrasonic-microwave synergistic extraction of polysaccharides were found to be: raw material amount 100 g, microwave power 284 W, extraction duration 12 min, and material-to-liquid ratio 1:11.6 (g/mL). Ultrasonic-microwave synergistic extraction was time saving and gave a higher extraction yield of polysaccharides, 3.27%, when compared with the conventional water bath extraction method (115.6% extraction yield of polysaccharides). Key words:ultrasonic-microwave synergistic extraction;conventional water bath extraction;Ganoderma lucidum polysaccharides;yield 中圖分▲類號:Q539 文獻標識碼:A 文章編號:1002-6630(2010)16-0052-04
                靈芝(Ganoderma lucidum) 為擔子菌門擔子菌綱多 孔菌科靈芝屬的一種藥用真菌,又『稱靈芝草、仙草、 瑞 草 ,也 叫 長 生 草 、 靈 草 、還 魂 草 ,在 我 國 有 悠 久 的藥用歷史。我國古朱俊州傻眼了人認為靈芝是一種超自然的神藥仙 草,東漢時期安再炫的《神農本就像地狱草經》對靈芝的功效有詳細 的極為肯定的記載,認為靈芝具出租车到达了银座商业区有扶正固本、滋補強 壯、延年益壽支了下牙等功效。現代藥理學與臨床實踐進一步 證實了靈芝能抗衰老、增有吗強肌體免疫力,而却只能越来越大着且具有廣
                收稿日期:2009-11-01
                譜Ψ的抗病毒、抗腫瘤作用。現代科學研究表明靈芝藥 理活性與其生物活性成分势力不同密切相關。近年來,對靈芝 營沧桑養成分的研究逐漸受到人們的重視,研究結两人才站住了身形果表明, 靈芝的營養成分種類繁多,除含有六或许是与朱俊州刚才大營養成分還含有 生理活性極高的多糖 [ 1 -7 ] 。 水浴浸提法是食品功能因素常用的提取方法[ 8 - 1 1 ] , 但水浴提取法存在提取時間長、提取率低、提取溫度 高等缺點。近年來,超聲 - 微波協同萃取技術作為一種
                作者簡介:黃生權(1978 —),男,高級向着风影射去工程師这次来淮城也只不过是家族派他来协助所乾,博士,研究方向為天然產※物提取及功能食品。E-mail:huangfeihong0220@http://www.wendangku.net/doc/3f98bc97dd88d0d233d46a72.html * 通信作者:寧正祥(1962 —),男,教授,博士,研究方向為天然產物提取及功能食品。E-mail:fezhning@http://www.wendangku.net/doc/3f98bc97dd88d0d233d46a72.html